Open Access Short Research Article

Synthesis of New Thiosemicarbazone Derivatives Substituted with Heterocycles with Potential Antitumor Activity

Patrícia S. Barbosa, Dayane S. S. De Souza, Maria D. Rodrigues, Karla M. Marques, Teresinha G. Da Silva, José G. De Lima

Asian Journal of Applied Chemistry Research, Page 1-7
DOI: 10.9734/ajacr/2018/v1i49695

Aims: To find new thiosemicarbazone derivatives with antitumor activity and good physico-chemical parameters.

Study Design:  A preliminary SAR study with pyrrolidinyl, piperidinyl, and morpholinyl heterocycles as well as hydrogen, alkyl and phenyl groups in the thiosemicarbazone scaffold.

Place and Duration of Study: Department of Pharmaceutical Sciences and Department of Antibiotics, from Federal University of Pernambuco (Brazil), between January 2016 and February 2017.

Methodology: The thiosemicarbazones were synthesized through a condensation reaction between benzaldehyde substituted with heterocycles and several thiosemicarbazides, using a catalytic amount of hydrochloric acid. The new compounds were characterised using classical spectroscopic and spectrometric techniques. The cytotoxicity were evaluated using MTT assay against five cancer cell lines and doxorubicin as reference compound. The LogP was also predicted using the software MarvinSketch 17.4.3 and promising results were found.

Results: We found five new thiosemicarbazone derivatives with excellent cytotoxicity.

Conclusion: Thiosemicarbazone derivatives are promising compounds in medicinal chemistry field and certainly should be considered as scaffold for drug candidates.

Open Access Original Research Article

Amino Acid Profile and Mineral Content of Balanites aegyptiaca Kernel

I. U. Muhammad, A. J. Alhassan, Y. Ramatu, A. Mohammad, A. Nasir, A. I. Yaradua, I. Alexander, Y. Umar, M. D. Ezema

Asian Journal of Applied Chemistry Research, Page 1-7
DOI: 10.9734/ajacr/2018/v1i49683

Study on the evaluation of the nutritional quality of poorly utilized plant products is of immense importance. This research work, therefore, evaluates the amino acid profile and mineral content of Balanites aegyptiaca kernel using standard analytical methods. The amino acid profile analysis revealed the presence of essential and the non-essential amino acids of which Total sulphur and Total aromatic amino acids content were 2.86 g/100 g protein and 7.21 g/100 g protein respectively. Analysis of the mineral content showed the presence of the following minerals in the order; Magnesium ˃ Sodium ˃ Calcium ˃ Potassium ˃ Iron ˃ Zinc ˃ Manganese ˃ Lead ˃ Copper ˃ Nickel ˃ Chromium ˃ Cadmium. The kernel could, therefore, be used as a good source of protein and minerals in supplementing other sources in order to reduce food insecurity and hence malnutrition from lack of protein diets.

Open Access Original Research Article

Assessment of Quality Parameters of Ecofriendly Biolubricant from Waste Cooking Palm Oil

M. U. Dabai, F. J. Owuna, M. A. Sokoto, A. L. Abubakar

Asian Journal of Applied Chemistry Research, Page 1-11
DOI: 10.9734/ajacr/2018/v1i49691

The use of vegetable oils as a renewable source for the production of ecofriendly biolubricant is gaining the attention of the renewable energy researchers and lubricating oil producers. This study evaluates the quality assessment parameters of ecofriendly biolubricant from waste cooking palm oil (WCPO). The crude WCPO was filtered, centrifuged at 500 rpm, and dried over Na2SO4 crystals overnight. The quality assessment parameters of the pretreated WCPO (PWCPO) were determined to authenticate its potential for the production of multigrade lubricating oils. Kinematic viscosities at 100°C (8.26±0.03 cSt) and 40°C (36.98±0.01 cSt) were determined according to ASTMD-446 method, while the viscosity index (208±0.11) was determined according to ASTMD-2270 method. A design of experiment (Mixture Design Method using Minitab 17) was used to determine the proportion of PWCPO (68.75%), SN 500 (23.75%), and additives (7.50%) that gave the mixture with the optimum quality parameters of the produced biolubricant. The produced biolubricant had kinematic viscosities at 100oC (10.72±0.13 cSt) and 40°C (59.32±0.20 cSt) respectively, a viscosity index of 173±0.10, flash point of 234±1.13°C, pour point of -31±0.10°C, acid value of 21.04±1.21 mg KOH g-1, and iodine value of 1.28±1.40 mg I2 g-1. The produced biolubricant has quality parameters that are comparable to available ecofriendly lubricating oil and was also found within standards for engine oils.

Open Access Original Research Article

Development of Stability Indicating and Robust RP-HPLC Method for Determination of Teneligliptin

Anvesha Vinit Ganorkar, Rekha S. Jibhkate, Krishna Radheshyam Gupta

Asian Journal of Applied Chemistry Research, Page 1-12
DOI: 10.9734/ajacr/2018/v1i49701

A simple and rapid reverse-phase HPLC method was developed for determination of Teneligliptin (TGP) in the presence of its degradation products generated from forced decomposition studies. The HPLC separation was achieved on a C18 ACE column (150x 4.6 mm i.d.; 5 μm) using mobile phase as a mixture of Phosphate buffer pH-7.2 using ortho-phosphoric acid: methanol (30:70v/v). The UV detection was carried out at 245nm at ambient temperature and the flow rate of 1.0 mL/min. The calibration curve was found to be linear in the concentration range of 10-50 μg/mL(r=0.9993). Force degradation study was performed under various conditions like acidic, alkaline, oxidative, photolytic and mass balance calculations were carried out from the degradation results. The developed method was validated as per ICH guidelines with respect to linearity, accuracy, precision, limit of detection and quantification. The robustness of the proposed method was evaluated by the Plackett Burman design. The purity of the degraded sample was checked by peak purity analysis. The peaks of degradation products did not interfere with that of pure Teneligliptin.

Open Access Original Research Article

Synthesis, Antibacterial and Antioxidant Activities of Some Tridentate Substituted Salicylaldimines

Abidemi Iyewumi Oloyede-Akinsulere, Jelili Olalekan Babajide, Simon Olonkwoh Salihu

Asian Journal of Applied Chemistry Research, Page 1-10
DOI: 10.9734/ajacr/2018/v1i49706

Five substituted tridentate salicylaldimines, (E)-2-((2-hydroxybenzylidene)amino)phenol, (E)-2-(((2-hydroxyphenyl)imino)methyl)-4-nitrophenol, (E)-4-chloro-2-(((2-hydroxyphenyl)imino)methyl) phenol, (E)-2-(((2-hydroxyphenyl)imino)methyl)-4-methoxyphenol, (E)-4-bromo-2-(((2-hydroxy-phenyl)imino)methyl)-6-methoxyphenol were synthesised and characterised by elemental analysis, IR, UV and NMR (1H and 13C). They were screened against some multi-drug resistance Gram-positive (Streptococcus agalactiae and Staphylococcus aureus), and Gram-negative (Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium) organisms by the agar-well diffusion method. The total antioxidant capacities of the salicylaldimines were determined by the phosphomolybdenum assay. Their antibacterial and antioxidant activities were screened to understand the substituents effects. The result showed that the methoxy-substituted compound exhibited the highest antibacterial and antioxidant activities while the nitro-substituted compound exhibited the least activities. This implies that the electron-donating group of the compound increases its antibacterial and antioxidant activities. The one-way analysis of variance was performed with MINITAB 17 at 95% confidence level.