Asian Journal of Applied Chemistry Research

  • About
    • About the Journal
    • Submissions & Author Guideline
    • Articles in Press
    • Editorial Policy
    • Editorial Board Members
    • Reviewers
    • Propose a Special Issue
    • Reprints
    • Subscription
    • Membership
    • Publication Ethics and Malpractice Statement
    • Digital Archiving Policy
    • Contact
  • Archives
  • Indexing
  • Publication Charge
  • Books
  • Testimonials
Advanced Search
  1. Home
  2. Archives
  3. 2018 - Volume 2 [Issue 2]
  4. Original Research Article

Submit Manuscript


Subscription



  • Home Page
  • Author Guidelines
  • Editorial Board Member
  • Editorial Policy
  • Propose a Special Issue
  • Membership

HPLC, Densitometric and Spectrophotometric Methods for the Simultaneous Determination of Colchicine and Probenecid in Their Binary Mixture

  • Samah Abd Elsabour Mohammed
  • Sawsan Abd El Moneim Abd El-Razeq
  • Israa Abd Elgafar Mohammed

Asian Journal of Applied Chemistry Research, Page 1-12
DOI: 10.9734/ajacr/2018/v2i229673
Published: 14 January 2019

  • View Article
  • Download
  • Cite
  • Statistics
  • Share

Abstract


Aim: To develop methods with  complete validation according to ICH guidelines and to be  applied for the determination of both drugs in laboratory prepared mixtures and in pharmaceutical formulation


Study Design: High performance liquid chromatography (HPLC), densitometric and different spectrophotometric methods (zero order, derivative ratio, ratio difference and mean centering) are developed for simultaneous determination of colchicine and probenecid in their combined pharmaceutical formulation. 


Methodology: High performance liquid chromatography separation is developed using C18 column and methanol: ammonia (100: 1.5 v/v) as a mobile phase. The densitometric method based on the separation of both drugs using chloroform: methanol: ethyl acetate: water: ammonia (7: 5:2.5:0.5:0.5 by volume) as mobile phase and scanning λ at 254 nm. Zero order determination is based on measurement of colchicine absorbance at 349 nm.  The first derivative ratio of peak amplitudes at 367 nm& at 290.4 nm and the ratio difference with the amplitude difference between (385 nm and 362.4 nm) and ( 270 nm and 255 nm)  for colchicine and probenecid, respectively are developed for the determination of both drugs. Mean centering determination of probenecid is developed by measurement at 279 nm using 3.6 µg/mL of colchicine as a divisor.


Results: HPLC method was applied over the  concentration ranges of 1.0-45.0 µg/mL & 0.5-30.0, while densitometric method was  linear over the concentration 0.15. 0-0.6 & 0.15-0.45 µg / band  and spectrophotometric methods were linear over the concentration ranges 10.00-55.0 & 3.6-20.0 µg/mL  for colchicine and probenecid, respectively.


Conclusion: Novel, simple and accurate method for the determination of colchicine and probenecid simultaneously in their binary mixture.


Keywords:
  • Colchicine
  • probenecid
  • HPLC
  • densitometry
  • spectrophotometry
  • Full Article - PDF
  • Review History

How to Cite

Mohammed, S., El-Razeq, S. A., & Mohammed, I. (2019). HPLC, Densitometric and Spectrophotometric Methods for the Simultaneous Determination of Colchicine and Probenecid in Their Binary Mixture. Asian Journal of Applied Chemistry Research, 2(2), 1-12. https://doi.org/10.9734/ajacr/2018/v2i229673
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
  • Abstract View: 1144 times
    PDF Download: 1115 times

Download Statistics

Downloads

Download data is not yet available.
  • Linkedin
  • Twitter
  • Facebook
  • WhatsApp
  • Telegram
Make a Submission / Login
Information
  • For Readers
  • For Authors
  • For Librarians
Current Issue
  • Atom logo
  • RSS2 logo
  • RSS1 logo


© Copyright 2010-Till Date, Asian Journal of Applied Chemistry Research. All rights reserved.