Characterization of Chemical Constituent and Biological Activity of Roots from Cleistanthus oblonggifolius (Roxb.) Mull. Arg
Asian Journal of Applied Chemistry Research,
Background: C. oblongifolius is a species of Asian trees, originally described by William Roxburgh and later placed by Johannes Müller Argoviensis; it is now included in the family Phyllanthaceae. Distributed throughout Indochina and Malesia, its name in Vietnam is cọc rào; it has been recorded from the Andaman & Nicobar Islands, Australia (Queensland), Bangladesh, Borneo, Cambodia, Java, Lesser Sunda Islands, peninsular Malaysia, Maluku, Myanmar, New Guinea, Philippines, Solomon Islands, Sulawesi, Sumatera, Vietnam and Thailand. Several species of this genus are highly poisonous (C. collinus), some are brewed for treating dysentery (C. decurrens), and used for treatment of asthma (C. myrianthus).
Objective: This study was conducted to investigate the effect of crude extract and purified substance on inhibition of AIDS and cancer.
Methods: Phytochemical studies were extracts from these solvents used for the study: hexane, ethyl acetate and methanol. The dried herbs were ground and extracted by chromatography. Extracts and compound have been tested for HIV-1 RT, anti-syncytium and cytotoxicity. The obtained purification compounds were used to determine the structure by spectroscopic techniques such as UV. IR, NMR and MS.
Results: The study found that compound 3-O-methylellagic acid 4'-O-alpha-L-rhamnopyranoside from the roots of this plant, it was also found that ethyl acetate and methanol class extracts inhibited anti-HIV-1 RT up to 100% and 65% pure substance. For all extracts and purified compounds, syncytium inhibition assay showed inhibition HIV with EC50 <7.8 µM, TI>2.31. SH-SY5Y anticancer was showed with ED50 = 6.7 µM.
Conclusion: C. oblongifolius extract, most of these fractions were potent in inhibiting HIV-1 RT and syncytium (MC99+1A2) inhibition. In addition, hexane extract inhibited the growth of SH-SY5Y cancer cells more than other cancer cells. However, the isolated purified compound only had an inhibitory effect on HT-29 cells with ED50 10.11 µg/mL.
- Cleistanthus oblonggifolius
- biological activity
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