Analytical Method Development and Validation for Estimation of Silymarin in Tablet Dosage form by HPLC

Main Article Content

Sneha Singh
Mohit Saini
Jitender K. Malik
Amit Kumar

Abstract

Silymarin is extracted from the Silybum marianum (milk thistle) plant C25 containing flavonoid mixture. It is mainly used for its effect in liver disease. The HPLC of silymarin tablet had been validated for precision, accuracy (recovery), selectivity & Linearity. In the present study, an attempt was made to provide a newer, simple, sensitive, precise and low cost HPLC method for the effective quantitative determination of silymarin as an active pharmaceutical ingredient as well as in pharmaceutical preparations without the interferences of other constituent in the formulations. HPLC method is developed and validated for various parameters as per ICH guidelines. The validated method was effectively useful to the commercially accessible pharmaceutical dosage form, yielding extremely good and reproducible result.

Keywords:
Silymarin, HPLC, precision, linearity, accuracy

Article Details

How to Cite
Singh, S., Saini, M., Malik, J. K., & Kumar, A. (2020). Analytical Method Development and Validation for Estimation of Silymarin in Tablet Dosage form by HPLC. Asian Journal of Applied Chemistry Research, 5(3), 22-31. https://doi.org/10.9734/ajacr/2020/v5i330135
Section
Original Research Article

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