Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

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Halima A. Umar
Hamidu Usman
Mustapha B. Abubakar
Baba F. Mohammed
Mohammed Babakura
Mohammed A. Sule

Abstract

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

Keywords:
Phyllanthus amarus, fractionation, fragmentation, soxhlet extraction, spectroscopy

Article Details

How to Cite
Umar, H. A., Usman, H., Abubakar, M. B., Mohammed, B. F., Babakura, M., & Sule, M. A. (2019). Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn. Asian Journal of Applied Chemistry Research, 2(3-4), 1-10. https://doi.org/10.9734/ajacr/2018/v2i3-430079
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Original Research Article