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In this study, the powder of Allcemilla vulgaris was used in the sythesis of silver and zinc nanoparticle. Biologically synthesized nanoparticles were characterized using Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, X-ray diffraction (XRD) and Zeta potential and then evaluated for antibacterial potential using micro dilution broth method. The minimum inhibitory concentration values of AgNP were 4.25 µg/mL and 6.64 µg/ mL for Escherichia coli O157:H7 and Staphylococcus aureus, respectively. Similarly, the MIC values of ZnNP were 3.32 µg/mL and 6.25 µg/mL, respectively for Escherichia coli O157:H7 and Staphylococcus aureus.